Tag Archives: Kent

Spring ephemeral ponds at Breakneck Creek

Christine, an undergraduate Biology student, set out to collect invertebrates in ephemeral ponds of varying longevities at Breakneck Creek, Kent.  Here are some photos of her sites.  The yellow/greenish dusting over the water is pollen from surrounding trees: apparently pollen was heavy in North Eastern Ohio, just as it was record breaking in Atlanta this Spring.

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Acid hydrolysis

Because 60 small (50 mL) flasks look cool.

Add concentrated sulfuric acid and produce an oxidized slurry of organic matter.

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Dilute the acid with distilled water, cover and autoclave the slurry

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Pre-weigh glass fiber filters

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Vacuum-filter slurry onto the pre-weighed filters

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Dry and reweigh filters.

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